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HydroShear- The best way to fragment DNA for library construction GeneMachines HydroShear offers the simplest, most reproducible, and most controllable method available for generating random DNA fragments. This software-driven device uses hydrodynamic shearing forces to fragment DNA strands. With the HydroShear, virtually any source of DNA at any concentration in volumes as small as 40μl can be randomly fractured to within a two-fold size distribution. For instance, if the desired target size is 2kb, the HydroShear can be calibrated to produce a distribution centered on 2kb with 90% of the DNA falling between 1.3kb and 2.6kb – ideal for plasmid sequencing libraries. Furthermore, because of the reproducibility of the HydroShear process, the distribution will be the same each day even with multiple users.
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