Olympus FV1200 Confocal

The FLUOVIEW FV1200 offers excellent precision, enhancing sensitivity and stability with an advanced optical system, scanners and detection system. In combination with the IX83 microscope, the FV1200 provides users with new opportunities in live cell imaging.

Imaging of living tissues demands the highest levels of sensitivity which allows for reduced laser power, phototoxicity and photobleaching, and innovative approaches to measurement of fluorescent indicators. The new FV1200 offers increased scanhead light throughput, cooled high sensitivity detector technology, and capitalizes on the advantages of the recently introduced IX83 automated inverted microscope platform. Enhancements in detector and coating technology allow for up to five simultaneous fluorescent detection channels. The industry’s first dedicated laser light stimulation scanner (SIM Scanner) achieves simultaneous stimulation and imaging for real-time visualization of rapid cell responses. This coordination of laser stimulation and imaging makes the FV1200 an ideal choice for FRAP, FLIP and photoactivation.

Specifications:

Laser Units

  • Automatic Introduction Optic    
  • AOTF (Single or dual fiber type)
  • Continuously variable
  • (0.1 % - 100 %, 0.1 % increment)
  • REX: Capable of laser intensity adjustment and laser wavelength selection for each region.
  • Optional Visible Light Laser for Stimulation       
  • LD Laser for Visible Light
  • 405 nm: 50 mW
  • 440 nm: 25 mW
  • 473 nm: 15 mW
  • 559 nm: 15 mW
  • 635 nm: 20 mW
  • Multiline Argon Laser (457 nm, 488 nm, 515 nm, Total 30 mW)
  • HeNe(G) Laser (543 nm, 1 mW)  \

Scanning and Detection 

  • Main Scanner
  • Standard Laser Ports: 3
  • Detector:
  • Standard: 3 Confocal Channels (3 Photomultiplier Detectors)
  • Cooled GaAsP-PMT 2 CH         
  • Optional 4 CH   
  • Photo Detection Method
  • Analog Integration      
  • Hybrid Photon Counting
  • VIS - UV - IR Excitation Dichromatic Mirror Turret: 6 positions (High Performance DMs and 20/80 Half Mirror)
  • Beamsplitter Turrets: 6 positions with CH1 and CH2
  • Wavelength Selection           
  • <Spectral type>
  • CH1 and CH2 equipped with independent grating and slit for fast and flexible spectral detection
  • Selectable wavelength bandwidth: 1 – 100 nm
  • Wavelength resolution: 2 nm
  • Wavelength switching speed: 100 nm/ ms
  • CH3 with 6 positions barrier filter turret
  • <Filter type>
  • CH1 to CH3 each with 6 positions barrier filter turret (High performance filters)
  • Galvanometer Scanner (Normal Imaging)
  • Galvanometer Mirror Scanner (X, Y): Dual Scanner (Anti-Oxidization Silver Coating)
  • Scanning Modes
  • 2D      
  • <Spectral type>: XY, XZ, XT, Xλ
  • <Filter type>: XY, XZ, XT
  • 3D      
  • <Spectral type>: XYZ, XYT, XYλ, XZT, XTλ, XZλ
  • <Filter type>: XYZ, XYT, XZT
  • 4D      
  • <Spectral type>: XYZT, XZTλ, XYTλ
  • <Filter type>: XYZT
  • 5D      
  • <Spectral type>: XYZTλ
  • <Filter type>
  • Other: Line scanning: Straight line with free orientation, free line, Point scanning
  • Scanning Speed         
  • 512 x 512 (1.1 s, 1.6 s, 2.7 s, 3.3 s, 3.9 s, 5.9 s, 11.3 s, 27.4 s, 54.0 s)
  • Bidirectional Scanning: 256 x 256 (0.064 s, 0.129 s), 512 x 512 (0.254 s)
  • Pinhole
  • <Spectral type>
  • Single motorized
  • Diameter ø50 – 300 μm (1 μm step)
  • <Filter type>
  • Single motorized
  • Diameter ø50 – 800 μm (1 μm step)
  • Scanning Zoom         
  • 1 X - 50 X in 0.1 X increments
  • Field Number (NA): 18
  • Z-Drive                  
  • Integrated motorized focus module of the microscope
  • Minimum increment: 0.01 μm or 10 nm
  • Transmitted Light Detector Unit: Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation to microscope frame

Microscope

  • Upright Frame                  
  • BX61
  • BX61WI
  • Inverted                
  • IX83 (IX83P2ZF)

Software

  • Image Acquisition       
  • Normal scan:
  • 64 x 64, 128 x 128, 256 x 256, 320 x 320, 512 x 512, 640 x 640, 800 x 800, 1024 x 1024, 1600 x 1600, 2048 x 2048, 4096 x 4096
  • Clip rectangle scan ,Clip ellipse scan ,Polygon clip scan,line scan ,free line scan,Point scan, Real-time image
  • Programmable Scan Controller: Time Controller function
  • 2D Image Display                        
  • Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input
  • 3D Visualization and Observation                        
  • Interactive volume rendering: volume rendering display, projection display, animation displayed (save as OIF, AVI or MOV format)
  • Free orientation of cross section display
  • 3D animation (maximum intensity projection method, SUM method)
  • 3D and 2D sequential operation function
  • Image Format                   
  • OIB/ OIF image format
  • 8/16 bit gray scale/index color, 24/ 32/ 48 bit color, JPEG/ BMP/ TIFF/ AVI/ MOV image functions
  • Olympus multi-tif format
  • Spectral Unmixing: 2 Fluorescence spectral unmixing modes (normal and blind mode)
  • Image Processing                       
  • Filter type: Sharpen, Average, DIC Sobel, Median, Shading, Laplacian
  • Calculations: inter-image, mathematical and logical, DIC background leveling
  • Image Analysis: Fluorescence intensity, area and perimeter measurement, time-lapse measurement
  • Statistical Processing: 2D data histogram display, colocalization
  • Optional Software:
  • Review station software, Off-line FLUOVIEW software for date analysis.
  • Motorized stage control software, Diffusion measurement package, Multi stimulation software, Multi area time-lapse software
  • Image Acquisition Mode                         
  • Map Image Acquisition                  
  • Multi Area Time-Lapse                  
  • Image Acquisition Area                  
  • Image Display                   
  • Cross Talk Reduction                    
  • Acquisition Image File Type                      
  • Image File Type Available for Viewing                    
  • Image Editing                   
  • 3D Image Construction                  
  • Basic Features                  
  • IR Laser Controlling                     
  • Optional Motorised-Stage Software                     
  • Optional Mapping and Multiplepoint Simulation Software                        
  • Optional Sequencer Manager

Manufacturer:Olympus
Model No:FV1200
Model No 2:Biological Confocal Laser Scanning Microscope
Product ID:12372aa

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