Microplate Reader

Molecular Devices SpectraMax M5 Microplate Reader

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Model No: 25038

Serial No: MV07183

Product ID: 13226

Microplate Readers: Molecular Devices, Inc.: SpectraMax M5 The upgradable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Your applications, your modes, your choice. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax M5 Microplate Reader possesses the five modes preferred by the majority of our users providing the benefit of multiple detection modes in one platform. Features: Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5 Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET and TR-FRET assays. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. If the needs of the researcher change, the SpectraMax M5 Microplate Reader can be upgraded with HTRF certified detection making it equivalent to the SpectraMax M5e Microplate Reader. Read Modes: • UV-Visible Absorbance (Abs) • Fluorescence Intensity (FI) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) • Glow Luminescence (Lumi) Applications: • ELISA, enzyme kinetics • DNA, RNA & Protein quantification ADME-Tox • Proliferation , Cell viability • Protease assays • IMAP kinase profiling • Intrinsic tryptophan fluorescence • GFP/YFP/CFP/dsRed • TRF and TR-FRET • Reporter Gene Assays • Cell-Migration/Chemotaxis Technical Specifications: - Primary detection modes: FI, FP, UV/VIS Abs, TRF, Lum - Wavelength selection: Tunable monochromator - Wavelength range (Abs): 200-1000 nm - Wavelength range (Fluorescence): 250-850 nm - Bandwidth: 4 nm for Abs 9 nm Ex, 15 nm Em for FI - Cuvettes: Abs, FI, Lum - Microplate formats: 6-384 - Cuvettes & test tubes: Yes - Endpoint read: Yes - Kinetic read: Yes - Multiple wavelength per well: Yes - Wavelength selectable luminescence: Yes - Spectral scanning: Yes - PathCheck Sensor: Yes - Temperature control: Yes (ambient +2 C to 60 C) - Plate handler: SynchroMax ET - Bottom-reading: FI, TRF, Lum - FDA 21 CFR Part 11 compliance: Yes - Robot compatible: Yes

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