Microplate Reader

The PHERAstar^®FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, and dedicated AlphaScreen^® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.

PHERAstar FSX

• Detection Modes
• Fluorescence intensity - incl. FRET
• Fluorescence polarization
• AlphaScreen^®/AlphaLISA^®/AlphaPlex^TM
• Luminescence (flash and glow) - incl. BRET
• Time-resolved fluorescence
• TR-FRET
• UV/Vis absorbance spectra
• Measurement Modes
• Top and bottom reading
• Endpoint and kinetic
• Sequential multi-excitation
• Sequential multi-emission
• Simultaneous Dual Emission
• Spectral scanning (absorbance)
• Real-time ratiometric measurements
• Well scanning
• Microplate Formats
• Up to 3456-well plates, user-definable
• LVis Plate with 16 low volume microspots (2 μL)
• Microplate Carrier
• Robot compatible
• Light Sources
• High energy xenon flash lamp
• Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex^TM
• Dedicated laser for TRF and TR-FRET
• Detectors
• Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes 
• CCD spectrometer
• Wavelength Selection
• Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application) 
• For top and bottom reading
• UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well
• Optical Path Guides
• Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
• Z-Adjustment
• Automatic focal height adjustment (0.1 mm resolution)
• Spectral Range
• Filters 230 - 750 nm or 230 - 900 nm for FI, FP
• 230 - 750 nm for LUM
• 230 - 900 nm for TRF Spectrometer 220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm 
• Sensitivity
• FI
• (top) < 0.15 pM FITC (384sv black, 20 μL)
• < 0.5 pM FITC (1536 black, 8 μL) FI
• (bottom) < 1.0 pM FITC (384g black, 50 μL) FP < 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
• < 1.5 mP SD at 1 nM FITC (1536 black, 8 μL) TRF
• with laser < 5 fM europium (384, 80 μL)
• < 15 fM europium (1536, 8 μL)  HTRF^®
• (black and white microplates) Reader Control Kit (Eu) after 18h (384sv, 20 μL)
• Delta F > 1100 % (High Calibrator)
• Delta F > 25 % (Low Calibrator) LUM < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
• Dynamic Range: 9 decades AlphaScreen^®
• with laser < 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL) ABS
• with spectrometer Selectable spectral resolution: 1, 2, 5, and 10 nm
• OD range: 0 to 4 OD; photometric resolution: 0.001 OD
• Accuracy: < 1% at 2 OD
• Precision: < 0.5% at 1 OD and < 0.8% at 2 OD 
• Linearity: ≤ 0.8% at 2.0 OD
• Read Times
• Flying mode
• (1 flash) 14 s (384)
• 27 s (1536) 
• 10 flashes 38 s (384)
• 1 min 52 s (1536) 50 flashes 1 min 29 s (384)
• 5 min 18 s (1536) 
• Reagent Injection
• Up to 2 built-in reagent injectors
• Injection at measurement position (6 to 384-well)
• Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
• Variable injection speed up to 420 µL / s
• Up to four injection events per well
• Reagent back flushing
• Shaking
• Linear, orbital, and double-orbital with user-definable time and speed
• Integrated Barcode Reader
• Up to three integrated barcode readers
• Incubation
• +5 °C above ambient up to 45 °C
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• The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
• Software
• Multi-user Reader Control and MARS Data Analysis Software included 
• FDA 21 CFR Part 11 compliant
• Dimensions
• Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
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