Microplate Reader

BioTek Instruments Synergy 2 Microplate Reader

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Model No: SL

Serial No: 266258

Product ID: 11166

Microplate Readers:Multi-Mode: BioTek Instruments: Synergy 2 The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. The dedicated absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. And, by utilizing dedicated optical elements for each individual detection technique, Synergy 2 offers uncompromised performance and the lowest limits of detection. Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance. Its three broad-spectrum light sources have been chosen for optimal illumination and excitation in all applications. General Detection mode Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa®, Luminescence, UV-Visible Absorbance Read method End point, kinetic, spectral scanning, well-area scanning Microplate types 6- to 1536-well plates (luminescence 1-384) PCR plates Compatible with Take3 Multi-Volume Plate with 2 µL microspots Temperature control 4°C above ambient to 65°C; +0.5°C at 37°C Shaking Yes Software Gen5™ included Control through USB or serial port Automation Yes AlphaScreen/AlphaLISA Light source Tungsten Halogen Lamp Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate Dynamic range >6 decades Reading speed 2 minutes typical for 96-well plate Detection system Ultra Low Noise PMT Absorbance Light source SQ Xenon Flash Wavelength selection Monochromator Wavelength range 200 - 999 nm, 1 nm increment Bandpass 2.4 nm Dynamic range 0 - 4.0 OD Resolution 0.0001 OD Pathlength correction Yes Monochromator wavelength accuracy +2 nm Monochromator wavelength repeatability +0.2 nm OD accuracy <1% at 2 OD typical <3% at 3.0 OD typical OD linearity <1% from 0 to 3.0 OD typical OD repeatability <0.5% at 2.0 OD typical Stray light 0.03% at 230 nm typical Reading speed 96: 11 seconds 384: 22 seconds 1536: 42 seconds Fluorescence Intensity Sensitivity Fluorescein 1 pM typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) - Top Fluorescein 5 pM typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) - Bottom Light source Tungsten Halogen High Energy DPR Xenon Flash (optional) Wavelength selection Deep blocking bandpass filters/dichroic mirrors Wavelength range 300 - 700 nm with tungsten lamp (850 nm option) 200 - 700 nm with xenon lamp (850 nm option) Dynamic range >6 decades Bandpass Filter dependent Detection system PMT Reading speed 96: 11 seconds 384: 22 seconds 1536: 42 seconds Luminescence Sensitivity 10 amol ATP typical (flash) Wavelength range 300 - 700 nm Dynamic range >6 decades Detection system Low noise PMT Fluorescence Polarization Light source Tungsten Halogen High Energy DPR Xenon Flash (optional) Sensitivity 3 mP at 1 nM fluorescein typical Wavelength selection Deep blocking filters/mirrors Wavelength range 400 - 700 nm (320 - 850 nm option) Time-Resolved Fluorescence Light source High Energy DPR Xenon Flash Sensitivity Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate) Wavelength selection Deep blocking filters/mirrors Wavelength range 200 - 700 nm (850 nm option) Reagent Dispensers Number 2 syringe pumps Dispense volume 5 - 1000 µL in 1 µL increment Dead volume 1.1 mL, 100 µL with backflush Physical Characteristics Power 250 Watts max Dimensions 17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm) Weight 60 lbs (27 kg)

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