BioTek Instruments MultiFlo FX Dispenser

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Model No: MFX

Serial No: 14111413

Product ID: 12862

MultiFlo™ FX is an automated reagent dispenser for 6- to 1536-well microplates. Its unique Parallel Dispense™ technology allows up to four independent reagents to be dispensed in parallel without potential carryover. What makes MultiFlo FX “multi-mode” is its configurability: you can add a wash module or the RAD™ module, for random access individual well dispensing and bulk dispensing larger wells. Everything you need for fast, intuitive programming and operation is available through the color touchscreen. MultiFlo FX configured with either the wash module or RAD technology replaces up to five liquid handlers in a single compact platform.


Multi-mode dispensing can replace up to four dispensers and a washer

Parallel Dispense™ technology: peristaltic and/or syringe pumps

RAD™ technology for dispensing to individual wells

Cost savings through low prime volume and single tube access

Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers

Walkaway automation with BioStack 4, for lidded or unlidded plates


Microplate types

Dispensing (peri pump and syringe)

96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)

Washing

96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)

Onboard software (computer control) 

Create, edit or run multiple protocols

Software LHC2 Software

LHC2 Secure for 21 CFR Part 11 compliance (option)

SiLA Compliant driver (option)

Shaking Programmable up to 60 minutes

Slow, medium, fast or variable

Soaking Programmable up to 60 minutes

USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard

Automation BioStack and 3rd party automation compatible

BioSpa 8 Automated Incubator compatible 


Washing

Manifold types 96- and 384-well washing: 8-tube manifold

6-, 12-, 24-, 48-well washing: Custom manifolds available

Volume range 20-30,000 µL/well

Wash cycles 1-10

Supply bottle 2 L

Dispense accuracy +3%

Dispense precision 96-/384-well plates, 300 µL/well: <3% CV

6-well plates, 5560 µL/well: <5% CV 

Residual volume 96-well plate, 300 µL/well: < 2 µL/well

Wash speed 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds

Wash volume 20-30,000 µL/well

Waste bottle 2L

Flow rates 140-422 µL/well/second

Sterilization Chemical 


Dispensing - Peristaltic pump (Multi-Channel)

Manifold types 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing

Fluid delivery 1 or 2 peristaltic pumps

Dispense speed

96 wells, 5 µL cass, 10 µL/ well: 3 seconds

96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds

384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds

384 wells, 10 µL cass, 10 µL/ well: 8 seconds

384 wells, 1 µL cass, 1 µL/ well: 6 seconds

1536 wells, 1 µL cass, 1 µL/ well: 21 seconds

Volume range 500 nL - 3,000 µL/well, selectable in 1 µL increments

Flow rates User programmable rates from high to low

Dispense performance

1 µL cassette: recommended range: 1 - 50 µL

Accuracy: +5% at 1 µL

Precision: <5% CV at 1 µL

<10% CV at 500 nL

Minimum Prime Volume: 1.20 mL


5 µL cassette: recommended range: 5 - 2,500 µL

Accuracy: +2.0% at 5 µL

Precision: <2.5% CV at 5 µL

Minimum Prime Volume: 4.23 mL


10 µL cassette: recommended range: 10 - 3,000 µL

Dispense Accuracy: +2.0% at 10 µL

Dispense Precision: <2.0% CV at 10 µL

Minimum Prime Volume: 7.36 mL

Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well

5 µL Cassette: 1,000 96-well microplates at 50 µL well

10 µL Cassette: 1,000 96-well microplates at 100 µL well

Sterilization Autoclave, chemical 


Dispensing - Syringe pump

Manifold types

96- and 384-well dispensing:

One 16-tube (2 x 8) manifolds - 316 SS tubes

Two 16-tube (1 x 16) manifolds - 316 SS tubes


1536-well dispensing:

Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 SS tubes

6- to 48-well dispensing: custom autoclavable manifolds available


Fluid delivery Two positive displacement syringe drives

Dispense speed

20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds

20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds

3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds

Volume range 3 - 3,000 µL/well selectable in 1 µL increments

Minimum Prime Volume: 12 mL

Flow rates User programmable rates from high to low

Dispense accuracy +1 µL at 5 µL

+1 µL at 20 µL

+1% at 100 µL

Dispense precision <5% CV at 5 µL

<2.5% CV at 20 µL

<1% CV at 100 µL

Supply bottle 1 L or 2 L

Sterilization Chemical, autoclavable option 


Dispensing - Peristaltic pump (RAD Technology)

Labware types

Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile

standard height and deep well formats; PCR trays and microtubes

8-to-1 tip: 6-, 12-, and 24-well plates

Manifold Types

RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle

RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute

Volume range 500 nL - 30,000 µL

Minimum prime volume

1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL

5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL

10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL

Dispense speed (high flow)

1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)

1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)

5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)

5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)

10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)

10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)

Dispense performance

1 µL cass (med), 0.5 µL/well: Precision 10% CV

1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV

1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV

5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV

5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV

10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV

10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV

8-to 1 cass(high), 40 µL/well: Accuracy + 4%

8-to 1 cass (high), >10 µL/well: Precision 2.5%CV

8- to 1cass(high), >80 µL/well: Accuracy + 2% 


Physical Characteristics

Power 100 - 240 Volts AC. 50/60 Hz

Dimensions Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm)

Weight Base instrument:

19.5 lbs (8.8 Kg) 

Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available. 

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