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Automated Cell Viability Analysis Today's research and biologic production demands on cell and tissue culture assumes no guesswork to confirming viable cell populations. Therefore, both cell concentration and percentage of viable cells must be determined accurately and precisely. The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer. The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.
Automation of the standard trypan blue assay % Viability Total cell concentration Total viable cell concentration Mean cell size Real time cellular images Bioprocess monitoring Validated reagents Convenient reagent pack
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