BD Biosciences LSRFortessa SORP 3 Lasers: 4Blue/2Violet/3YellowGreen Flow Cytometer

Recent PM from BD (11/16/22); Certified and Ready for a Service Contract! 

Includes:

• HTS (High Throughput Options)
• Windows 7 Pro computer with FACSDiva Software Version 8.0.1
• Installed Lasers:
• Violet Laser 405 nm: 50 mW with 2 PMT detectors (Trigon)
• 525/50 (505LP)
• Z473/10
• Blue 488 nm: 50 mW with 3 PMT detectors plus side scatter (Octagon)
• 710/50 (685LP)
• 582/15 (570LP)
• 488/10
• 675/20 (670LP)
• 515/20 (505LP)
• Yellow Green 561 nm: 50 mW with 3 PMT detectors (Octagon)
• 610/20 (600LP)
• 670/630 (635LP)
• 582/15

Technical Specifications:

• Excitation Optics
• Excitation Optical Platform:The special order BD LSRFortessa optical layout allows for up to five lasers.
• Flow Cell Design:Rectangular Quartz Cuvette: Internal cross-section, 430 x 180 μm
• Fixed optical assembly of the lasers, with up to 5 spatially-separated laser beams.

Emission Optics

• Optical Coupling:The quartz cuvette flow cell is gel coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
• Forward Scatter Detection:Photodiode detector with a 488/10 bandpass filter
• Side Scatter Detector:Photomultiplier tube (PMT) with a 488/10 bandpass filter
• Emission Optical Design:The special order BD LSRFortessa cell analyzer uses BD’s patented octagon and trigon detection system. Using reflective optics and fiber-coupled PMTs ensures more efficient light collection than that obtained from transmission optics.

Performance

• Fluorescence Sensitivity:FITC-A: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE-A: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
• Measurements performed using SPHERO Rainbow Calibration Particles RCP-30-5A
• Fluorescence Resolution:Coefficient of variation PI: area of <3%, full G0/G1 peak for chicken erythrocyte nuclei (CEN) stained with propidium iodide (PI)
• Fluorescence Linearity:Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
• Side Scatter Sensitivity:Achieves separation of 0.5-µm beads from noise in side scatter.
• Forward and Side Scatter Resolution:Scatter performance is optimized for resolution of lymphocytes, monocytes, and granulocytes.
• Forward Scatter PMT Option:A forward scatter PMT upgrade is available for small particle detection through the special order program.
• Data Acquisition Rate:Theoretical maximum electronic data collection of 70,000 events/second.
• Typical operation at 20,000 events/second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min

Fluidics

• Sample Flow Rates:Front key panel provides three modes: RUN, STNDBY, and PRIME
• Continuously adjustable flow rate, plus three preset flow rates:LO = 12 μL/min
• MED = 35 μL/min
• HI = 60 μL/min
• Standard Fluidic Reservoirs:One 8-L sheath and 10-L waste container provided.